Abstract
The aim of the study is to investigate the influence of diet treatment on bone marrow cells. Normal male Wistar rats were divided into six groups (n = 6 per group): control with normal diet (C), increased fructose (31 % w/w in fodder) (Fr) and high fatty (30 % w/w of animal fat in fodder) diet (Fa), and the same diets with vanadium complex ([VO(4,4′ Me2-2,2′ Bpy)2]SO4) · H2O (CV, FrV and FaV). During 5 weeks, the animals had unlimited access to food and water. Immediately after anaesthetizing and sacrificing the animals, bone marrow smears were prepared from the femurs. Different types of cell lines in the animal smears were examined under the microscope: erythroid line, myeloid line, monocytic line, megakariocytic line and lymphoid line. Addition of fructose or animal fat had evident influence on the proportional composition of the bone marrow cells. In erythroid precursors, addition of both investigated products resulted in a statistically significant increase of percentage of this type of cells. A reverse effect was observed for the lymphoid cell line where addition of both tested diets decreased quantity of these cells in comparison to the control diet. In the same lines, addition of vanadium intensified the observed changes. In the case of other types of cell lines, statistically significant changes were not observed.
Highlights
Bone marrow is an indispensable structure in the vertebrate organism
Mean body mass growth was highest in the group with fructose diet and lowest in the vanadium-treated control group
In the case of groups consisting of animals not treated with vanadium, the lowest consumption was observed in the group with fatty diet and highest in the control group
Summary
Bone marrow is an indispensable structure in the vertebrate organism. It plays two important roles: produces all blood cells and participates in immunological reactions of the organism. Cells at different stages of development are present in the bone marrow. In the case of rats, the main cell line present in marrow is myeloid—it usually amounts to 30–50 % of all cells. Other lines: monocytic, megakariocytic and lymphoid constitute a lower percentage [1,2,3]
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