Abstract

Studies were carried out on the changes in solubility in SDS+β-mercaptoethanol, the –SH group content, the amino acid and the fatty acid compositions, and nutritional quality of the muscle protein following freezing, storage at −20°C and conventional defrosting of sardines (Clupea pilchardus). During the processes, 8% of the total SH– group content was lost and there was a slight drop in solubility in SDS+β-mercaptoethanol. The amino acids which dropped to the greatest extent were the S-amino acids, followed by histidine, tyrosine, leucine, lysine and phenylalanin. The C22:6/C16:0 ratio dropped and this was considered to be related to oxidation phenomena. The digestibility of protein, biological value and net protein utilization (NPU) also dropped as a result of the processes studied.

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