Influence of foetal calf serum supplementation during in vitro embryo culture in Iberian red deer.

Abstract

Nowadays, the use of foetal calf serum (FCS) during in vitro embryo culture is very controversial. Whilst some authors have encouraged its use, others reject it because of its harmful effects. Although in vitro embryo production in red deer is a promising assisted reproductive technique, it is still in its infancy and a great effort is needed to update the protocols used. The aim of this study was to assess whether FCS supplementation in red deer embryo culture medium is necessary to produce blastocyst and, if so, when is the best time to add it in terms of blastocyst production and quality. In vitro blastocysts were cultured with FCS added at 24, 48 or 96hours post-insemination (hpi). In addition, a treatment without FCS was used as control. Six hundred and ninety-four cumulus-oocyte complexes were collected for in vitro fertilization. Cleavage rate was examined at 48hpi, and blastocyst yield was recorded on days 6, 7 and 8. FCS had no influence on cleavage and blastocyst rate for any of the treatments studied. However, the number of cells was higher (p=.025) in those blastocysts cultured with FCS from 48hpi compared with FCS-free culture media (93.88±7.76 vs. 54.11±8.36). In conclusion, the addition of FCS to the embryo culture medium at 48hpi improves the quality of red deer blastocyst, although it does not affect the percentage of embryos obtained.