Abstract
AbstractThe characteristics of Pseudomonas fluorescens biofilms formed under three different linear flow velocities (u=0.1, 0.4, and 0.8 m/s; Reynolds numbers of 1,000, 4,000, and 8,000, respectively) were studied providing an extension to the studies of earlier researchers. A flow cell reactor system was used to form biofilms, and they were characterized in terms of thickness, morphological structure, mass, cell density, outer membrane protein expression, and matrix and total protein and polysaccharide content. The external mass transfer coefficients were also assessed. The biofilms developed at u=0.4 and 0.8 m/s had similar characteristics but were significantly different from those developed at 0.1 m/s. High flow velocities formed thinner biofilms with higher cell densities and contents of matrix/extracellular proteins and polysaccharides. The increase of flow velocity from 0.4 to 0.8 m/s caused a higher production of matrix proteins and polysaccharides. The external mass transfer coefficients sugg...
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