Abstract
SummaryWe investigated the dependence of in vitro rooting and acclimatisation to greenhouse conditions on the source of iron used in the shoot multiplication and rooting media using five raspberry (Rubus idaeus L.) cultivars (‘Beskid’, ‘Canby’, ‘Malling Seedling’, ‘Norna’, and ‘Veten’). Ethylenediamine di-2-hydroxy-phenyl acetate ferric (FeEDDHA) in the rooting medium led to higher chlorophyll contents, earlier and more abundant rooting (8.7 vs. 5.3 roots per shoot), 30% higher fresh and dry weights, and thus higher quality microplants than ethylenediaminetetra-acetate ferric sodium (FeEDTA). Higher quality microshoots had a beneficial effect on acclimatisation (i.e., percentage survival and length of shoots) when the microplants were planted in a peat-based substrate; however, when planted in vermiculite, the initial differences disappeared during a 4 week-long growth period in a greenhouse.
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