Abstract
The cultural microbiomes of 27 bacteria colonies were isolated from Mugil cephalus for analysis of the antibacterial and antagonistic activities. A potent probiotic bacterium was characterized using16S r RNA sequencing. The potent strain was added to fish diet to perform the challenge test and to study the growth and immunological parameter. The extracellular proteins from the probiotic were collected and characterized using MALDI TOF/TOF. Out of G27, G9 strain inhibited all the five pathogenic strains. An isolated bacterium was identified as Bacillus subtilis PRBD09 with accession number KF765648. After 35 days of feeding period B. subtilis PRBD09 enhance the both cellular and humoral immune responses, which responsible for survive of the Mugil cephalus against Aeromonas hydrophila infection. The MALDI TOF sample 08 and 09 were recognized as hypothetical proteins based on the MALDI TOF sample. A cytidinedeaminase was found in samples 10, 11, and 12. Extracellular proteins may be involved for the immunological increase in Mugil cephalus against Aeromonas hydrophila, according to the current research.
Highlights
The cultural microbiomes of 27 bacteria colonies were isolated from Mugil cephalus for analysis of the antibacterial and antagonistic activities
Twenty seven (G1-G27) bacteria isolated was obtain in the intestine of fish Mugil cephalus
The antibacterial activity of the 27 bacterial strains isolated from the gut of Mugil cephalus showed different zone of restraint against various fish pathogens such as such as A. hydrophila, V. alginolyticus, V. harveyi, V. parahaemolyticus, V. vulnificus (Table 1)
Summary
The cultural microbiomes of 27 bacteria colonies were isolated from Mugil cephalus for analysis of the antibacterial and antagonistic activities. A potent probiotic bacterium was characterized using16S r RNA sequencing. The extracellular proteins from the probiotic were collected and characterized using MALDI TOF/TOF. After 35 days of feeding period B. subtilis PRBD09 enhance the both cellular and humoral immune responses, which responsible for survive of the Mugil cephalus against Aeromonas hydrophila infection. Extracellular proteins may be involved for the immunological increase in Mugil cephalus against Aeromonas hydrophila, according to the current research. Probiotic was most important feed supplement for control or treatment to the disease of fish in the a quaculture[5]. Maintain and attachment sites, adhere to the gut, and inhibit pathogen growth for nutrients and valid alternative to prophylactic antibiotics and b iocides[9]. Fish feed with probiotics which enhance the growth and disease resistant[10]. While use of Probiotic fish feeds in the aquaculture is new concept[11]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
