Abstract
A model system was established to determine whether plasmid transfer occurs in soil and how various environmental conditions and cellular energy states affect the rate of plasmid transfer. Different donor and recipient bacteria were inoculated into sterile sandy lutitic soil microcosms. Dispersion studies were performed with a multipoint inoculator sampler. Transconjugant cells were enumerated by direct plating on antibiotic-amended LB medium. The influences of soil moisture (6.7 to 60%), incubation temperature (4° to 44°C) and pH (5.3 to 9.2) on cell dispersal and on plasmid transfer were examined. Maximum transfer frequencies were observed at: 20% of moisture content, pH between 7 and 8, and 30°C. These results indicate that plasmid transfer may occur in soil and that environmental conditions may significantly affect the rate of transfer.
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