Abstract

Commercial reagents are often contaminated wit encentrations which may affect biological immunoassays. The endotoxin lipopolysaccharide from E. coli 026:B6, at concentrations at and above 5×10 −9 g/ml induces mononuclear cells to produce the lymphokine leucocyte migration inhibition factor (LIF). However, lipopolysaccharide at concentrations at or above 5 1;0 −8 g/ml inhibit leucocyte migration making it impossible to measure the effect of LIF. Standardization of endotoxin content during production and assay of LIF is needed, in order to reduce the variability of the results.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.