Influence of ejaculatory abstinence on seminal total antioxidant capacity and sperm membrane lipid peroxidation

Abstract

To determine whether the period of ejaculatory abstinence (EA) influences the total antioxidant capacity (TAC) of semen or lipid peroxidation (LPO) of sperm membranes. A prospective experimental trial. Academic medical center for reproductive endocrinology and infertility. Forty men from infertile couples planning intrauterine insemination. Men provided semen specimens after EA periods of 1 and 4days. Semen analysis, peroxidase staining, and assays for seminal TAC and sperm membrane LPO, with measures compared between days 1 and 4 within individuals (internal control) using paired t tests. The shorter period of EA (1day vs. 4days) resulted in statistically significant decreases in semen volume (-24%), sperm density (-28%), and total sperm count (-3.2%). There was a statistically significant increase in TAC with the shorter period of EA (1day) compared with 4days of EA. No difference was detected in sperm membrane LPO comparing 1day of EA and 4days of EA. Higher seminal TAC obtained after a shorter period of EA could diminish oxidative stress-induced sperm damage by amechanism independent of LPO. Shorter periods of EA may thus improve sperm quality by protecting from reactive oxygen species damage, even though lower numbers of motile sperm are produced after a shorter period of EA. This would be consistent with prior research indicating improved results after intrauterine insemination under these circumstances.