Influence of edaravone on major organ functions and plasma inflammatory cytokine level following hemorrhagic shock and resuscitation in rats

Abstract

Objective To investigate the effects of edaravone on the functions of major organs and plasma inflammatory cytokine levels following hemorrhagic shock(HS) and resuscitation in rats.Methods A total of 24 rats were randomly divided into 3 groups: the sham-operated control group(sham),the haemorrhage shock goup(I/R),and the haemorrhage shock plus edaravone injection(I/R-ED) group.Rats in the latter two groups were bled using a femoral artery catheter with MAP maintained within 35-45 mmHg(1 mmHg=0.133 kPa) for 60 min to induce shock,and then resuscitaton was induced by reinjecting 60% autologous blood and 2 times of shed blood Ringer's.At the beginning and ending of resuscitation,I/R group and I/R-ED group were given the same dose(2 ml/kg) of normal saline and edaravone,respectively.The plasma levels of TNF-α,IL-6,ALT,AST,LDH,CK,BUN and Cr were measured at 2 and 24 h after resuscitation.Results(1)The plasma levels of TNF-α and IL-6 in I/R group were significantly higher than those of the sham group 2 h after hemorrhagic shock and resuscitation(P0.05),with no significant difference found between I/R group and I/R-ED group(P0.05).The plasma levels of TNF-α and IL-6 in I/R group were also significantly higher than those in the sham group after 24 h(P0.05),and the levels in I/R-ED group was significantly lower than those in the I/R group(P0.05).(2) The plasma levels of ALT and AST in I/R group were significantly higher than those in the sham group at 24 h(P0.05),and the levels in I/R-ED group was significantly lower than that in the I/R group(P0.05).The plasma levels of BUN and Cr in I/R group were significantly higher than those in the sham group after 2 h(P0.05),and the levels in I/R-ED group was significantly lower than those in the I/R group(P0.05).The plasma levels of BUN and Cr in I/R group were also significantly higher than those in the sham group(P0.05);Cr level in ED group was significantly lower than that in the I/R group(P0.05);and the BUN level in I/R-ED group was similar to that in I/R group.(3)Great pathological changes were found in the liver,kidney,and lung of rats in I/R Group,and the pathological changes were slight in I/R-ED Group.Conclusion Our study suggests that edaravone can decrease the release of inflammatory factors(TNF-α and IL-6) after induction of HS in rats,which can alleviate the pathological damage to major organs after HS.