Influence of dl-ethionine on the nad content and on the activity of the poly(adpr)synthetase in the rat liver

Abstract

1. 1. Ethionine, an analogue of methionine, is known to cause a number of serious changes. In this study, its influence on the NAD + NADH 2 content and on the activity of the poly(ADPR)synthetase in rat liver has been investigated. 2. 2. dl-Ethionine decreases the content of NAD + NADH 2 in adrenalectomized, but not in normal animals. 3. 3. After injection of nicotinamide the NAD + NADH 2 content in the liver of adrenalectomized rats is much higher than in that of normal ones. 4. 4. DL-Ethionine diminishes the NAD synthesis caused by nicotinamide in adrenalectomized animals and increases it in normal ones. dl-Tryptophan induces only a slight NAD synthesis, which is reduced in adrenalectomized animals by ethionine. 5. 5. 1-Methylnicotinamide slightly decreases the NAD concentration in normal animals, but increases it considerably in adrenalectomized ones. dl-ethionine reduces the NAD + NADH content in adrenalectomized animals in the presence of 1-methylnicotinamide. 6. 6. The activity of poly(ADPR)synthetase of in-vitro tested nuclei is slightly increased after treatment with dl-ethionine.