Abstract
Excised cotton bolls were inoculated with an aflatoxin-producing strain of Aspergillus flavus (ATCC #26994), and were placed in growth chambers for 7 or 3 days with short (2-hour) or long (10-hour) diurnal maximum temperature cycles. The maximum and minimum temperatures were 30-32 C and 16-18 C, respectively. The percent bright greenish-yellow (BGY) fluorescence of locks and seeds, as well as seed infection of BGY-fluorescent seeds, increased as the duration of the daily maximum temperature of 30 C increased, and/or as the number of diurnal maximum temperature cycles of 30 C increased. Aflatoxin did not accumulate in A. flavus-infected seed, regardless of treatment.
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