Influence of Dihydroquercetin, 1-Aryltetrahydroisoquinoline (F-18) and Their Conjugate (DHQ-11) on Mitochondrial Respiration and Oxidative Phosphorylation

Abstract

In current scientific article, rat liver mitochondria respond to the flavonoids dihydroquercetin (20–100 µM), 1-(2´-bromo-4´,5´-dimethoxyphenyl)-6,7-dimethoxy-1,2,3,4 for respiratory and processes of oxidative phosphorylation - tetrahydroisoquinolines F-18 (20-100 μM) and synthesized on the basis of followings 2-(3,4-dihydroxyphenyl)-6-[1-(2'-bromo-4',5'-dimethoxyphenyl)-6,7- The effect of dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl]methyl-3,5,7-trihydroxychroman-4-on the concentrations of the DHQ-11 conjugate (20-100 μM) was investigated in <i>in vitro</i> experiments. Experiments were conducted within 180-200 gram infertile white male rats. Mitochondrias from rat liver were isolated by the way of differential centrifugation. Concentrations of dihydroquercetin flavonoid 20, 60, 100 μM in FAD-dependent succinate oxidation in rat liver mitochondria were identified to be reliable respiratory control parameters and partially increased ADF/O value, the respiratory control coefficient as Chans demonstrated an increase in respiratory rate under the influence of the alkaloid F-18 isoquinoline 20, 60, 100 μM. Moreover, the ADP/O coefficients also boosted under the influence of the isoquinoline alkaloid F-18. It was observed that ADP/O ratio coefficients was expanded within the bonding by the effect of the DHQ-11 conjugate concentration. The concentration of 100 μM dihydroquercetin for FAD-dependent succinate oxidation in rat liver mitochondria surged Chance's respiratory rate by 15% compared to the control. Concentrations of isoquinoline alkaloid F-18 100 μM enlarged the respiratory rate by 13% compared to the control. It was defined that concentrations of 100 μM DHQ-11 conjugate rose the respiratory rate by 20%. The oxidation of FAD-dependent substrates in mitochondria was more actively effected by the DHQ-11 conjugate than dihydroquercetin and F-18 isoquinoline alkaloids.