Abstract
Background: Platelet concentrates are routinely manufactured from whole blood by differential centrifugation. During this storage period under blood bank conditions, biochemical, structural and functional changes occur, a process that is also known as platelet storage lesion. Their quality was assessed using the following parameters: platelets, leukocytes and erythrocytes counts, pH, CD63, lactate dehydrogenase and Annexin V. Materials and Methods: In this experimental study, 25 platelet concentrates prepared with platelet rich plasma-platelet concentrates, 25 units via buffy coat and apheresis-derived platelet methods. The percentages of Annexin V, CD63 expression, lactatedehydrogenase, platelet, leukocytes counts and pH were evaluated. Results: During storage for up to 5 days, no significant pH, difference was observed among all three type of platelet concentrates (p>0.05). The mean leukocytes count buffy coat units, platelet rich plasmaplatelet concentrates and apheresis-derived units were comparable and statistically significant difference was observed (p<0.05). During storage for up to 5days platelet concentrates units displayed significant an increase in lactatedehydrogenase, CD63 and Annexin V expressions, as compared with buffy coat units and apheresisderived units preparation on day 5 (p<0.05). Discussion: The kinetics of CD63 and annexin V levels are influenced by the method used to prepare platelets for storage. The different levels of CD63, annexin V and lactatedehydrogenase in three types of units clearly demonstrating a progressive activation process of platelet concentrates units exceeds that of buffy coat and apheresis-derived units. Further clinical studies will be necessary to determine whether meet the quality criteria or is superior in predicting in vivo viability.
Highlights
Blood banks optimize their protocols to yield the highest quality products in the shortest possible time
The kinetics of CD63 and annexin V levels are influenced by the method used to prepare platelets for storage
The different levels of CD63, annexin V and lactatedehydrogenase in three types of units clearly demonstrating a progressive activation process of platelet concentrates units exceeds that of buffy coat and apheresis-derived units
Summary
Blood banks optimize their protocols to yield the highest quality products in the shortest possible time. Processing and storage may affect both platelet quality and function and they may be less effective when transfused than fresh platelets [7,8,9]. Platelet concentrates are routinely manufactured from whole blood by differential centrifugation. During this storage period under blood bank conditions, biochemical, structural and functional changes occur, a process that is known as platelet storage lesion. Their quality was assessed using the following parameters: platelets, leukocytes and erythrocytes counts, pH, CD63, lactate dehydrogenase and Annexin V
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