Influence of different concentrations of 6-benzylaminopurine and thidiazuron on the proliferative activity of apple varieties in in vitro culture

L.V Tashmatova,V.V Shakhov,O.V Matsneva,T.M Khromova,E Renev,V Romanyuk,R Changzhong,Y Shavrokov,G Batalova

doi:10.1051/bioconf/20213603012

Abstract

An essential role in the process of clonal micropropagation is played by cytokinins, with the help of which apical dominance is removed and the formation of additional shoots and buds is activated. The article presents the study results of microshoots’ proliferative activity of apple varieties on media with 6-benzylaminopurine at a concentration of 1.0 mg/l, 2.0 mg/l and thidiazuron at a concentration of 0.1 mg/l, 0.2 mg/l and 0.3 mg/l. The objects of research were apple varieties of the VNIISPK selection - Bolotovskoe, Imrus, Veteran, Kandil orlovsky, Girlyanda, Priokskoe. The aim of the research was to study the effect of substances with cytokinin activity on the morphogenesis of apple varieties. As a result of the research, it was revealed that both cytokinins and varietal characteristics influence the multiplication factor. The highest multiplication factor was observed when using 6-BAP. Thidiazuron reduced the proliferation rate. When using 6-BAP, Bolotovskoe, Girlyanda and Priokskoe varieties had the highest multiplication factor among the varieties. When using TDZ - varieties Bolotovskoe and Kandil Orlovsky.

Highlights

In the world industry, the apple tree occupies a significant place among pome crops
This problem can be solved with the help of clonal micropropagation, which consists in replicating plants under aseptic conditions on specially selected nutrient media and is used for accelerated propagation of varieties, hybrids, forms and species of plants, as well as for the production of planting material due to a high multiplication factor [1]
The main goal of the micropropagation stage is to obtain the maximum number of microshoots from each explant by sequential cultivation of existing microshoots on a fresh nutrient medium

Summary

Introduction

A topical issue is the establishment of healthy mother plantations of promising varieties. In this regard, there is a need for a sufficient amount of healthy planting material. It is usually carried out using preparations with cytokinin activity. The cytokinins' group includes both natural compounds (zeatin) and synthetic ones (6-benzylaminopurine, kinetin, 2isopentenyladenine, thidiazuron) [3]

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