Influence of Different Arsenic Species on the Bioavailability and Bioaccumulation of Arsenic by Sargassum horneri C. Agardh: Effects under Different Phosphate Conditions.

Abstract

The growth response and incorporation of As into the Sargassum horneri was evaluated for up to 7 days using either arsenate (As(V)), arsenite (As(III)) or methylarsonate (MMAA(V) and DMAA(V)) at 0, 0.25, 0.5, 1, 2, and 4 μM with various phosphate (P) levels (0, 2.5, 5 and 10 μM). Except As(III), algal chlorophyll fluorescence was almost similar and insignificant, regardless of whether different concentrations of P or As(V) or MMAA(V) or DMAA(V) were provided (p > 0.05). As(III) at higher concentrations negatively affected algal growth rate, though concentrations of all As species had significant effects on growth rate (p < 0.01). Growth studies indicated that toxicity and sensitivity of As species to the algae followed the trend: As(III) > As(V) > MMAA(V) ~ DMAA(V). As bioaccumulation was varied significantly depending on the increasing concentrations of all As species and increasing P levels considerably affected As(V) uptake but no other As species uptake (p < 0.01). The algae accumulated As(V) and As(III) more efficiently than MMAA(V) and DMAA(V). At equal concentrations of As (4 μM) and P (0 μM), the alga was able to accumulate 638.2 ± 71.3, 404.1 ± 70.6, 176.7 ± 19.6, and 205.6 ± 33.2 nM g-1 dry weight of As from As(V), As(III), MMAA(V), and DMAA(V), respectively. The influence of low P levels with increased As(V) concentrations more steeply increased As uptake, but P on other As species did not display similar trends. The algae also showed passive modes for As adsorption of all As species. The maximum adsorption of As (63.7 ± 6.1 nM g-1 dry weight) was found due to 4 μM As(V) exposure, which was 2.5, 7.3, and 6.9 times higher than the adsorption amounts for the same concentration of As(III), MMAA(V), and DMAA(V) exposure, respectively. The bioavailability and accumulation behaviors of As were significantly influenced by P and As species, and this information is essential for As research on marine ecosystems.