Influence of dietary lipid and carbohydrate levels and chronic 3,5,3′-triiodo-l-thyronine treatment on thyroid function in immature rainbow trout, Oncorhynchus mykiss

Abstract

The influence of varying dietary levels of nonprotein energy sources (lipid, L; carbohydrate, C) and 3,5,3'-triiodo-L-thyronine (T3) on thyroid function in immature rainbow trout was studied. Three diets of equivalent available energy content and identical nutrient composition, except for dissimilar concentrations of L and C (diet 1, L = 7%, C = 28.3%; diet 2, L = 13%, C = 14.9%; diet 3, L = 19%, C = 1.5%), were each supplemented with 0, 4, 8, or 12 ppm T3 and fed to satiation to trout at 6.5 +/- 0.5 degrees on a 12-hr photoperiod for 12 weeks. Dietary L and C concentrations did not influence plasma total L-thyroxine (T4) or T3 levels, indices of free T4 or free T3 levels, hepatic T4 5'-monodeiodinase (5'D) activity, capacity or affinity of hepatic nuclear T3 receptors, or thyroid follicle epithelial cell height. T3 treatment elevated total and free T3 levels and decreased 5'D activity (Vmax) in approximate proportion to T3 dose, and without effect on plasma total or free T4 levels or T3 receptor properties. However, thyroid follicle epithelial cell height was depressed at 8 or 12 ppm dietary T3. In trout reverted for 20 days to a T3-free diet from a T3 (12 ppm) diet, plasma total T3 levels fell to 30% of those of control trout (0 ppm T3 throughout). It was concluded that, under our experimental conditions, (i) trout thyroid function was refractory to dietary concentrations of L and C, (ii) the primary response to T3 supplementation was suppressed hepatic 5'D level and T3 production, which was sustained for at least 20 days after T3 treatment ceased, and (iii) despite causing a probable indirect decrease in thyroidal secretion, T3 did not modify the set point of the hypothalamo-hypophyseal-thyroid axis based on plasma total or free T4 levels.