Influence of diazotrophic inoculations on nitrogen nutrition of rice

Abstract

An experiment was conducted in microplots (7 by 7m) to investigate the effect of 2 non-symbiotic N2-fixing bacteria [Azotobacter (strain CS1) and Azospirillum (strain CM4)] in the presence of 50 kg N/ha on the performances of the diazotrophs with respect to nitrogen accretion and its transformation in the rhizosphere soils of rice (Oryza sativa L. cv. IR-36). In most cases, a successful inoculation of the diazotrophs was recorded, with the proliferation of Azotobacter and Azospirillum, either alone or in combination, in the rhizosphere soils, and nitrogenase activity (C2H2 reduction) of the microbes was present in rice roots. The uninoculated soil receiving 100 kg N/ha recorded the highest amount of total nitrogen, non-hydrolysable organic nitrogen, available nitrogen, and hydrolysable organic nitrogen content in the rhizosphere soils, resulting in greater yield of the crop. Inoculation of the diazotrophs substantially increased different fractions of nitrogen content in the rhizosphere soils, and the increase in total nitrogen, non-hydrolysable organic nitrogen, and hydrolysable organic nitrogen was greater due to Azotobacter than either Azospirillum or a combination of Azotobacter and Azospirillum. Total and mineral nitrogen content increased at maximum tillering to flowering stages of the crop, followed by a decline at maturity, whereas, hydrolysable organic nitrogen decreased with a concomitant increase in non-hydrolysable fraction with the age of the crop.