Abstract
SUMMARYThree novel dehydrated wheat/rice cereal functional products with an addition of well documented probiotic Bifidobacterium animalis ssp. lactis BB-12® (BB-12®) were developed in Podravka factory for the infants older than 4 months: instant rice cereal, instant rice cereal with fruits and instant wheat cereal with vanilla. Notably, the number of viable BB-12® cells in each of the novel products was higher than the required minimal number of probiotic cells per gram of product (106 CFU/g) during the storage period of 106 weeks. Therefore, BB-12® strain recovery and genome stability were evaluated by strain-specific polimerase chain reaction and amplified fragment length polymorphism fingerprinting analysis. Further aim was to evaluate the influence of these three different cereal food matrices on specific probiotic properties of BB-12® strain in vitro. Applied food matrices positively influenced the survival in the simulated conditions of the gastrointestinal tract and antagonistic activity against undesirable microorganisms, while no influence on auto- and coaggregation ability of B. animalis ssp. lactis BB-12® was observed. Adhesion to extracellular matrix proteins and intestinal epithelial Caco-2 cells together with antibacterial activity emphasized competitive pathogen exclusion from Caco-2 cells by probiotic strain BB-12®.
Highlights
Probiotics are living microorganisms that, when administered in adequate amounts, confer a health benefit to the host [1]
We evaluated the functional properties of probiotic strain Bifidobacterium animalis ssp. lactis BB-12® incorporated in dehydrated wheat/rice cereal products developed on a pilot scale, namely instant rice cereal, instant rice cereal with fruit and instant wheat cereal with vanilla, produced in Podravka d.d., Koprivnica, Croatia
polymerase chain reaction (PCR) with Bifidobacterium strain-specific primers and amplified fragment length polymorphism (AFLP) DNA fingerprints confirmed the presence of BB-12® strain in newly developed wheat/rice cereal infant products, where the determined probiotic bacterial counts were above 106 CFU/g, as requested for probiotic products by FAO/WHO
Summary
Probiotics are living microorganisms that, when administered in adequate amounts, confer a health benefit to the host [1]. Probiotic bacteria are mostly members of Lactobacillus and Bifidobacterium genera. These genera are members of indigenous microbiota of humans and animals and are generally recognized as safe (GRAS) [2]. An important probiotic property of bifidobacteria is the production of short-chain fatty acids (SCFAs), as well as breakdown of carbohydrates and proteins in the intestinal tract. It is believed that this feature is most valued because of the proven clinical effects of Bifidobacterium animalis ssp. Lactis BB-12® strain, in the prevention of diarrhoea caused by antibiotics, Clostridium difficile and rotavirus [3]. Strain BB-12® is one of the world's best-studied probiotic strains [4]
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