Influence of culture media and Trichoderma spp. on the growth and sporulation of Pseudocercospora griseola in vitro

Abstract

Angular leaf spot (Pseudocercospora griseola) impacts the productivity of bean crops (Phaseolus vulgaris). In culture media, the pathogen P. griseola requires nutritional, light, and temperature control for its growth and sporulation. For the management of angular leaf spot, it is estimated that biological control with Trichoderma species, through parasitism or production of antifungal metabolites, is efficient. This research sought to identify the best culture medium for the growth and sporulation of P. griseola and evaluate its interaction with Trichoderma spp, through antagonism and production of volatile and non-volatile metabolites. P. griseola sporulation was stimulated on oat-agar and bean leaf extract-agar media. The interaction between Trichoderma species and P. griseola had an antagonistic effect, and the metabolites produced by antagonist inhibited the growth and sporulation of the pathogen. These results indicate the potential of Trichoderma spp. for the management of angular leaf spot, offering an alternative to the use of fungicides.