Abstract
This work presents the experimental study results of the influence of the culture medium on the ability to IAA synthesis of three endophytic strains TH10R, TH11T, and TH13T from roots of Ipomoea pes-caprae. Three investigated strains give the highest IAA concentration after 96 h of cultivation. A significant increase in IAA biosynthesis was obtained by cultivating the TH10R strain in a medium containing lactose or starch as a carbon source and NH4Cl or KNO3 as a nitrogen source. The TH11T strain produces the maximum amount of IAA, using glucose or xylose and KNO3 or NH4NO3 as carbon and nitrogen sources, respectively. Sucrose is a suitable carbon source for the TH13T strain; on a sucrose-containing medium, the TH13T strain produces the highest IAA amount. The most active strain is TH10R, identified as Bacillus mycoides and named Bacillus mycoides TH10R.
Highlights
The term "endophyte" is broadly used for fungi, bacteria or other microorganisms that colonize living tissues of a plant without causing obvious pathological symptoms
Endophytic bacterial strains isolated from Ipomoea pes-caprae roots, TH10R, TH11T and TH13T are stored in the laboratory of the Department of Microbiological Biotechnology, Faculty of Biotechnology, Vietnam National Agricultural University
This study examined the effect of six different carbon sources on IAA production (Fig. 2)
Summary
The term "endophyte" is broadly used for fungi, bacteria or other microorganisms that colonize living tissues of a plant without causing obvious pathological symptoms. Interest in the use of biological products based on microorganisms that can stimulate the growth and development of plants and prevent the spread of infections is increasing in crop production [1]. One of these products is IAA (Indole-3-acetic acid) - the most common natural auxin that affects the most important physiological processes in plants. The plant has the ability to live in harsh and unstable environmental conditions due to a complex of adaptive properties, among which symbiosis with endophytic bacteria is among most important
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