Abstract
The “Fagioli di Sarconi” common beans, typical of Basilicata Region (Southern Italy), include different ecotypes protected by the European Union with the mark PGI (Protected Geographical Indication). The study aimed to determine the presence of seed-borne pathogens, isolated from two ecotypes of “Fagioli di Sarconi” common beans, “Ciuoto” and “Cannellino rosso”, in two different cultivation areas during the years 2018 and 2019, for identifying genotypes resistant and well adapted to climatic changes. The seeds were evaluated for seed-borne pathogens screening by using three validated seed health testing methods, according to the 2020 International Rules for seed testing. The washing test identified 18 fungal pathogens, different for ecotype and year of observation; the 1% sodium hypochlorite treatment strongly reduced the contaminants. With the blotter test, several saprophyte pathogens were found. Between paper test, specific for detecting the C. lindemuthianum, revealed the presence of this pathogen for both ecotypes and years, in all areas, and individuated some bacteria, too. In conclusion, this work highlighted differences by the two PGI common bean ecotypes in response to seed-borne pathogens resistance and environmental change due probably to their different thickness and polyphenolic content of integument.
Highlights
Phaseolus vulgaris L., known as common bean, is the second most important legume in the world for food purposes thanks to its high nutritional value in terms of content of protein, vitamins, zinc, iron, and fiber [1,2]
The “Fagioli di Sarconi” common beans, typical of Basilicata Region (Southern Italy), include different ecotypes protected by the European Union with the mark PGI (Protected Geographical Indication)
The study aimed to determine the presence of seed-borne pathogens, isolated from two ecotypes of “Fagioli di Sarconi” common beans, “Ciuoto” and “Cannellino rosso”, in two different cultivation areas during the years 2018 and 2019, for identifying genotypes resistant and well adapted to climatic changes
Summary
Phaseolus vulgaris L., known as common bean, is the second most important legume in the world for food purposes thanks to its high nutritional value in terms of content of protein, vitamins, zinc, iron, and fiber [1,2]. It is widespread over a large area between 52° N and 32° S of latitude, at low altitudes until sea level (USA and Europe) and high altitude (South America) [3]. The main seed-borne pathogenic fungi that cause losses of yeld and quality of common bean in South Italy are Colletotrichum lindemuthianum The study aimed to determine the presence of seed-borne pathogens, Proceedings 2020, 4, x; doi: FOR PEER REVIEW www.mdpi.com/journal/proceedings
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