Influence of CRBN, IKZF1, and IKZF3 expression on outcome in lenalidomide treated newly diagnosed multiple myeloma patients

Abstract

RB1,TP53, t(4;14), t(11;14), t(14;16) and a centromeric probes for chromosomes 7, 9, 11, 15 and 17 (Abbott-Vysis Inc., Downers Grove, IL, USA; Dako, Glostrup, Denmark; Kreatech, Amsterdam, The Netherlands; MetaSystems, Altlusheim, Germany). Some complex chromosomal rearrangements were analyzed by using mFISH and arrayCGH methods (MetaSystems;Agilent, Santa Clara,CA,USA). Results: Patients with the absence of delRB1 had significantly increased levels of sOPG (p 1⁄4 0.025) and sVEGF (p 1⁄4 0.036). The presence of t(11;14) was associated with lower levels of sIL-6R (p 1⁄4 0.009). The presence of t(14;16) was associated with higher levels of 2m (p 1⁄4 0.022), and lower serum levels of DKK-1 (p 1⁄4 0.023). The presence of gain 1q21 was associated with higher levels of sTK (p 1⁄4 0.026), sIL-6R (p 1⁄4 0.01), and lower serum levels of PINP (p 1⁄4 0.030). Conclusion: This showed that some cytogenetic changes have an impact on individual components of the cytokine network in MM. The presence of adverse cytogenetic changes is associated with higher levels of neoplastic mediators (sTK, sIL-6R, 2m) and a decrease in levels of bone formation parameters (sOPG, PINP and DKK-1). Identified data complete the image of biology of neoplastic processes in MM. Supported by IGA CR NT 12451/5, NT 14400, NT 14393