Abstract

Diagnosis of latent endometritis and predicting the effect of the inflammatory process on the course of pregnancy and its outcome is a necessary measure when conducting clinical examination of animals. Lack of diagnostic criteria, including microbiological one, cause difficulties for veterinarians in assessing latent endometritis. Often, animals suffering from latent inflammation of the reproductive tract are infertile and cannot be treated. And from cows with dysbiosis of the genital tract, sick young animals are born, lagging behind in growth and development. The aim of the work was to establish the relationship between the genital tract microbiome, the number and species composition of commensals and opportunistic microorganisms on the ability to carry pregnancy. Washes from the oral and nasal cavities were taken from the young born to study the microbiome: lactic acid microorganisms in combination with opportunistic pathogens. As a result of the research, it was found that animals with a low number of lactic acid microorganisms and an increased titer of opportunistic microorganisms were infertile, which led to their culling. In young animals, they are present in the respiratory tract in all samples, together with coccal microorganisms. The study of lactic acid microorganisms is advisable in the diagnosis of inflammatory processes of the reproductive tract and upper respiratory tract.

Highlights

  • Inflammations of cattle pelvic organs cause economic damage to agricultural enterprises due to early culling of cows, shortage or receiving sick non-viable young animals

  • When examining the microflora of the cervical canal of cows, it was found that bifidobacteria are found in an amount of in 7 samples, in 2 samples, in 5 samples; lactobacilli in the amount of were not found, - in 4 samples, - in 2 samples, - in 1 sample; microorganisms of the family Bacillus spp. cultivated in a dilution of in 4 samples, in a dilution of in 1 sample; E. coli without hemolytic properties was cultivated in 1 sample at a dilution of 102

  • A relationship has been established between the content of normal flora, CPB of the reproductive tract and the ability to reproduce: artificial insemination of animals with a quantitative content of CPB exceeding the amount of normal flora turned out to be ineffective

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Summary

Introduction

Inflammations of cattle pelvic organs cause economic damage to agricultural enterprises due to early culling of cows, shortage or receiving sick non-viable young animals. Absence of pronounced clinical manifestations, lead to the fact that latent inflammation of the pelvic organs is not provided with proper and timely treatment, and to intrauterine infection of calves. Diagnosis of latent endometritis can help to avoid culling of animals. Considering that this disease has a microbial etiology, it is necessary to take into account the species and biochemical properties of the microbial community when carrying out diagnostic measures. The aim of the study is to establish the relationship between the composition and properties of the microflora of the cows’ genital tract and the microflora biotypes’ colonization of the calves obtained from these cows

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