Influence of Corynebacterium pseudotuberculosis infection on level of acute phase proteins in goats

Z K H Jeber,F F Jesse,Z Mohdjin,H Wahid,A A Saharee,R Yusoff,J Sabri

doi:10.1186/s12917-016-0675-y

Z K H Jeber, F F Jesse + Show 5 more

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https://doi.org/10.1186/s12917-016-0675-y

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Abstract

BackgroundGoat caseous lymphadenitis (CLA) is a chronic disease caused by Corynebacterium pseudotuberculosis. However, there is paucity of data about goat’s acute phase response during the course of CLA. This study was conducted to investigate the response of acute phase proteins, mainly haptoglobin (Hp), serum amyloid A (SAA) and the negative acute phase response, especially albumin after an experimental challenge of C. pseudotuberculosis and phospholipase D (PLD) in Cross bred Boer goats.ResultsSerum Hp concentration in goats challenged with C. pseudotuberculosis (inoculated with 1x109 cfu subcutaneously) showed a significant increase, 5 fold in males (0.98 ± 0.12 mg/ml) and 3 fold in females (0.66 ± 0.12 mg/ml) compared to the control (0.2 ± 0.02 mg/ml). Challenge with PLD (1 ml/20 kg body weight intravenously) also showed significant increase, 4 fold in males and females (0.89 ± 0.11 mg/ml; 0.82 ± 0.12 mg/ml) respectively compared to the control (0.2 ± 0.02 mg/ml). Albumin concentration showed a significant decrease in both treated groups compared to the control. There were no significant changes in SAA concentration between challenged and control goats.ConclusionsThere was a significant response by Hp to C. pseudotuberculosis infection and PLD challenge. This was supported by the early acute response in which Hp was detected before CLA lesions were developed. Therefore, it concluded that C. pseudotuberculosis and PLD can influence the level of acute phase proteins in goats.

Highlights

Goat caseous lymphadenitis (CLA) is a chronic disease caused by Corynebacterium pseudotuberculosis
Haptoglobin Mean serum concentrations of haptoglobin showed significant increase (p < 0.05) in both C. pseudotuberculosis and phospholipase D inoculated groups compared with the unchallenged goats
Mean serum concentration of Hp in C. pseudotuberculosis inoculated males was higher than phospholipase D inoculated males; while it was the opposite in the females where mean serum concentration of Hp was significantly higher (p < 0.05) in phospholipase D inoculated females than C. pseudotuberculosis inoculated females (Table 1)

Summary

Introduction

Goat caseous lymphadenitis (CLA) is a chronic disease caused by Corynebacterium pseudotuberculosis. Results: Serum Hp concentration in goats challenged with C. pseudotuberculosis (inoculated with 1x109 cfu subcutaneously) showed a significant increase, 5 fold in males (0.98 ± 0.12 mg/ml) and 3 fold in females (0.66 ± 0.12 mg/ml) compared to the control (0.2 ± 0.02 mg/ml). Challenge with PLD (1 ml/20 kg body weight intravenously) showed significant increase, 4 fold in males and females (0.89 ± 0.11 mg/ml; 0.82 ± 0.12 mg/ml) respectively compared to the control (0.2 ± 0.02 mg/ml). Albumin concentration showed a significant decrease in both treated groups compared to the control. There were no significant changes in SAA concentration between challenged and control goats. Caseous lymphadenitis in goats distributed throughout almost the whole world. Most common manifestations are abscesses in the superficial lymph nodes of the body and less often

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