Influence of compound Danshen injection on expressions of nitric oxide, B-cell lymphoma-2 and S100 in septic shock rats with brain injury

Abstract

Objective To explore the influence of compound Danshen injection on the expressions of nitric oxide(NO), B-cell lymphoma-2(bcl-2)protein and S100 in the septic shock rats with brain injury. Methods Forty 2-week-old healthy Wistar rats were randomly divided into 4 groups, 10 cases in every group.Septic shock group 1(6 h)and septic shock group 2(12 h): the rats were injected with lipopolysaccharide (5 mg/kg) via the tail vein to make the septic shock models; and the treatment group: rats were intraperitoneally injected with compound Danshen injection (5 mg/kg) immediately when shock signs appeared; Normal control group: the rats were given the same amount of 9 g/L saline while the other groups received drugs; The septic shock group 2 were drawn 12 h after the successful mode-ling, while the remaining 3 groups were harvested at 6 h after the success of the modeling.The content of NO in the brain tissue was detected with nitrate reduction way, and the expression of bcl-2 in hippocampus was examined with immunohistochemistry technique, meanwhile the concentration of serum S100 was measured by enzyme linked immunosorbent assay. Results Compared with the normal control group, the contents of NO, bcl-2 and S100 in the septic shock 1 group increased significantly(F=25.64, 3 027.17, 632.04, all P<0.05); however in treatment group, the contents of NO and S100 were less than those in the septic shock groups, while the level of bcl-2 was more than that in the septic shock groups, and there were significant differences(F=25.64, 632.04, 3 027.17, all P<0.05); compared with the septic shock group 1, the contents of NO and S100 in the septic shock group 2 increased drastically, but the level of bcl-2 in the septic shock 2 group was decreased, and there were significant differences(t=-12.34, -4.89, 6.88, all P<0.05). NO and bcl-2 had significant negative correlation(r=-0.928, P<0.05); NO had significant positive correlation with S100(r=0.877, P<0.05). Conclusions NO participates in brain injury of septic shock rats, through inhi-biting bcl-2 expression and up-regulating S100 expression.Compound Danshen injection has a protective effect on the brain tissues of rats during septic shock, through reducing the contents of NO as well as S100 and elevating the expression of bcl-2. Key words: Compound Danshen injection; Septic shock; Nitric oxide; B-cell lymphoma-2 protein; S100