Abstract
Functional role of molybdenum as co‐factor in the production of bioplastic (polyhydroxyalkanoates [PHA]) was studied at different concentrations (in µM; 2.5 (M1); 5 (M2); 10 (M3); 15 (M4); 20 (M5); 25 (M6)) by using Pseudomonas otitidis. Designed synthetic wastewater (DSW) was used in all the experiments. Known amounts of acetate, propionic acid, and butyric acids were added to DSW and were used as substrate (carbon source). Dehydrogenase (DH) is one of the key enzymes in PHA production. Addition of molybdenum as co‐factor to DH enzyme resulted in maximum PHA production at M5 (69%) associated with higher DH activity (3.7 µg/mL). Low concentration of molybdenum (M1, M2) did not show any significant influence on PHA production. Highest molybdenum concentration (M6) used showed lower PHA production when compared to control. Produced polymer contained short chain length PHA, with co‐polymer composition of poly‐(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate), P(3HB‐co‐3HV). Substrate degradation rate (SDR) was evaluated at different concentrations of molybdenum. High SDR was registered at M5 due to high enzyme activity.
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