Abstract
Estimation of the energy exchange parameters during partial brain ischemia offers an opportunity for finding new ways of correcting the above pathology. The aim of the study was to investigate the changes in mitochondrial respiration in the homogenates of rats with brain cerebral ischemia of various duration. The experiment was carried out on 19 white female rats in whom the right common carotid artery was ligated for 1 hour or 3 days with subsequent decapitation and brain removal. The rate of mitochondrial respiration in mitochondrial suspension of brain homogenates was assessed by means of the polarographic method using succinate or a mixture of L-malate/L-glutamate and adenosine diphosphate as respiratory substrates. Ligation of the right common carotid artery of various duration resulted in reduced mitochondrial respiration rate which was more pronounced after 1 hour of partial brain ischemia as compared to 3 hours. Modeling of partial cerebral ischemia in rats by ligation of the right common carotid artery considerably inhibits respiratory activity of the brain mitochondria leading to the dissociation of oxidation and phosphorylation in the mitochondria and inducing impairment of neuronal bioenergetics in cerebral tissue due to the disturbance of the supply of oxygen by blood. Understanding of pathophysiological processes in mitochondria during partial brain ischemia will help to develop new methods of pathogenetic correction of changes in cell structures of the brain tissue. This approach offers new possibilities for the pathogenetic therapy.
Highlights
The relevance of cerebrovascular diseases of ischemic genesis is indisputable since they are associated with high morbidity, mortality and disability worldwide
There was a significant decline in the majority of mitochondrial respiration (MR) rates in the group of animals with 1 hour brain partial ischemia (BPI) compared with group of sham operated rats
Group of rats with 3 days partial cerebral ischemia (PCI) compared with sham operated animals had the tendency to decrease in V1 – by 13.4% (p > 0.05) and considerable drop in following parameters: V2 – by 17.4% (p < 0.05), V3 – by 25.6% (p < 0.05), V4 – by 18.5% (p < 0.05)
Summary
The relevance of cerebrovascular diseases of ischemic genesis is indisputable since they are associated with high morbidity, mortality and disability worldwide. Morbidity and mortality rates from stroke remain among highest in Belarus. According to the official statistics the incidence of strokes in the republic is 296 cases per 100,000 people [14], in the Russian Federation – 350-400 cases per 100,000 people [13], which is 2-2.5 times higher than in the European countries. The annual death-rate from ischemic stroke among the active population in the Republic of Belarus corresponds to 25.1% per 100 thousand population [2] and in the RF – 374 per 100 thousand people [12], which is 4 times as high as in Canada and USA [1]. According to the World Health Organization [15] stroke ranks second as a cause of death worldwide
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