Influence of Cation Porphyrins on DNA Damage during Irradiation by X-rays

Abstract

In this work, the effect of TOEPyP4 porphyrins on DNA damage induced by X-ray beams was investigated. The influence of X-ray radiation with different doses on DNA isolated from the calf thymus, as well as DNA/TOEPyP4 complexes, was investigated. To study the influence of the presence of porphyrins on radiation induced DNA damages with different relative concentrations of complexes were investigated (r = 0.01; 0.04, where r = Cporf./CDNA). Then an attempt was made to reveal the possible potentiating effects of porphyrins on DNA damage depending on the concentration of porphyrins and the radiation dose. The studies were carried out by the spectroscopy melting method in 10-3 M NaCl buffer solution, pH 7.2. From obtaining dates, the dependence of the protective or radiation-enhancing properties of porphyrins on the relative concentrations - irradiation dose combination is evident.