Influence of carbon-to-nitrogen ratio on the mixed-acid fermentation of wastewater sludge and pretreated bagasse

Abstract

In mixed-acid fermentation, carbon and nitrogen are critical nutrients for cell synthesis, growth, and metabolism. To study the effect of C/N ratio on the yield of carboxylic acids, wastewater sludge was co-digested with pretreated bagasse; the amount of sludge was varied from 0% to 100% (dry weight basis). Fermentation was performed at 55°C at a solids concentration of 50g dry solids/L, and Iodoform was used to inhibit methane formation. It was observed that C/N ratio significantly affects yield, especially at extreme ratios. The highest carboxylic acid yield (0.36g acids/g VS fed) was obtained for C/N ratios ranging from 13 to 25g C/g N. C/N ratio also affected the composition profile of carboxylic acids. In all mixtures, acetic acid was the major fraction, followed by butyric acid. However, i-butyric, valeric acid, and i-valeric acid increased with increasing sludge content, which likely resulted from protein degradation.