Abstract
1. 1. Ca/Na exchange in dog red cells is greatly stimulated by pretreatment of the cells with diamide, but only if the diamide preincubation is carried out in a Na-free, Ca-containing medium. 2. 2. Membranes prepared from dog red cells that had been pre-exposed to diamide in solutions containing various combinations of Ca and Na were subjected to polyacrylamide gel electrophoresis and Western blotting. 3. 3. The band representing glyceraldehyde-3-phosphate dehydrogenase was selectively increased under the very same ionic conditions that result in a diamide-induced stimulation of Ca/Na exchange. 4. 4. Glyceraldehyde-3-phosphate dehydrogenase may participate in the modulation by diamide of Ca/Na exchange in dog red cells.
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