Influence of biogenic metals nanoparticles on the morphogenetic processes effectiveness of mint peppery (Mentha piperita L.) in the culture in vitro

Abstract

Investigation of the influence of nanoparticles of copper and cobaltous on the micropropagation of plants of peppermint (M. piperita L.) in such varieties as «Mama», «Chornolist» and «Lydia» on the basis of a complex of culture methods of isolated tissues and organs in vitro is carried out. According to the research, it can be stated that there is the positive influence of the cobaltous and copper nanoparticles on the morphogenesis of peppermint explants in culture in vitro. After 28 days of cultivation in MurashigeSkoog growth medium (MS) with nanoparticles of copper with concentration of 0.8 mg/l there was a significant increase in the quantity of the bines of such varieties as «Mama» for 50%, «Сhernolistnaya» — 43%, in growth medium with addition of copper with a concentration of 1.2 mg/l the plants of peppermint of variety «Lydia» has the enlargement of 48% (p<0.05). The number of nodes in a nutrient medium with copper nanoparticles with a concentration of 0.8 mg/l was larger in comparison with controlled medium of variety «Mama» for 29% (p<0.05), «Сhernolistnaya» — 31% (p<0.05), and in the medium with copper nanoparticles with a concentration of 1.2 mg/l of mint plants of such variety as «Lydia» for 43% (p<0.05). The optimum density of cobaltous in the MS growth medium was identified. It is 0.4 mg/l and the optimum concentration of copper is 0.8–1.2 mg/l. The usage of such modification of Murashige-Skoog growth medium allows to receive more (an average 27%) regenerated peppermint by plants. After 28 days the coefficient of reproduction of peppermint plants of variety «Mama» was 1:14, «Lydia» — 1:13, «Сhernolistnaya» — 1:15.