Abstract
The aim of the study was to identify the effect of bacteriophage microbiota infection on the expression of alpha-synuclein (A-syn) in rat small intestinal wall cells.Material and methods. The work was performed on Wistar rats, which once a day rectally injected a cocktail of bacteriophages against pathogenic bacteria or sterile saline (control). Various parts of the small intestine were frozen on dry ice, and cryostat sections were prepared to immunohistochemically investigation the localization of A-syn in the cells of the intestinal wall after 10 days of experiment.Results. A significant quantitative increase of immunopositive to A-syn lymphocytes and an increase in the expression of this protein in the neurons of the intermuscular and submucous nerve plexuses were detected.Conclusion. Bacteriophage infection of the microbiota has a pronounced effect on the expression of alpha synuclein in lymphocytes and neurons localized in the wall of the small intestine.
Highlights
The aim of the study was to identify the effect of bacteriophage microbiota infection on the expression of alpha-synuclein (A-syn) in rat small intestinal wall cells
The work was performed on Wistar rats, which once a day rectally injected a cocktail of bacteriophages against pathogenic bacteria or sterile saline
Bacteriophage infection of the microbiota has a pronounced effect on the expression of alpha synuclein in lymphocytes and neurons localized in the wall of the small intestine
Summary
Цель работы – изучить влияние бактериофагального инфицирования микробиоты на экспрессию альфа-синуклеина (А-син) в клетках стенки различных отделов тонкой кишки крыс. Через 10 сут животных выводили из эксперимента, различные участки тонкой кишки замораживали на сухом льду и готовили криостатные срезы для иммуногистохимического выявления локализации А-син в клетках стенки кишечника. Через 10 сут ректального введения бактериофагов во всех изученных отделах тонкой кишки обнаруживалось достоверное количественное увеличение лимфоцитов, иммунопозитивных к А-син, и усиление экспрессии этого белка в нейронах межмышечных и подслизистых нервных сплетений. Измерение уровня А-син в плазме крови крыс экспериментальной группы показало достоверное увеличение концентрации этого белка относительно контроля. Более высокие уровни А-син в плазме крови животных экспериментальной группы могут быть следствием его повышенной продукции в стенке кишечника как лимфоцитами, так и нейронами ЭНС в условиях повышения кишечной проницаемости, вызванной дисбалансом микробиоты. The aim of the study was to identify the effect of bacteriophage microbiota infection on the expression of alpha-synuclein (A-syn) in rat small intestinal wall cells
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