Influence of B Vitamins on Proliferation and Differentiation of Osteoblastic Bovine Cell Cultures: An In Vitro Study

Kent Urban,Sebastian Bürklein,Ulrich Plate,Julia Auer

doi:10.1007/978-981-13-1002-7_13

Kent Urban, Sebastian Bürklein + Show 2 more

Open Access

https://doi.org/10.1007/978-981-13-1002-7_13

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Publication Date: Jan 1, 2018
Citations: 1	License type: CC BY 4.0

Affiliation: University of Münster

Abstract

Minerals and vitamins affect bone formation, genetics, nutrition, and hormones. Studies mainly focus on the elucidation of the metabolic pathways during biomineralization to get an idea of how to promote the process of biomineralization in vivo and in vitro. One qualified approach to reach this is to investigate the influence of different substances on the proliferation and differentiation of osteoblastic cell cultures in vitro. The aim of this study was to investigate the effects of different types of single B vitamins (B6, B9, and B12) and a vitamin B complex (B1, B2, B3, B5, B6, B9, and B12) on proliferation and differentiation of primary bovine osteoblastic cells in vitro. The proliferation of osteoblastic cells during the experiments was evaluated by cell number analysis while cultivating. The expression of marking proteins of the osteogenic differentiation was evaluated by immunohistochemistry. Previous experiments with seven different B vitamins in different concentrations revealed a positive effect on cell proliferation with increasing concentration caused by three B vitamins: pyridoxal (B6), folic acid (B9), and cobalamine (B12). The use of vitamin B6, B9, and B12 in different concentrations resulted in a significant increase of cell proliferation (p < 0.05). But neither the B vitamins nor the B vitamin complexes stimulated the expression of the typical bone cell proteins.

Highlights

Focussing on the rapid development of implant- and bone-substitute materials as well as their integration in autologous tissue and optimized wound healing processes, it gets more and more important to give well-known therapies new perspectives
For a better adaption of implants in surrounding tissue, material’s biocompatibility and bone regeneration as a part of wound healing becomes subject of scientific research
Many existing therapies on bone regeneration dealing with vitamin D and calcium supplementation are well established (Avenell et al 2014; Javed et al 2016; Vandenbroucke et al 2017)

Summary

Introduction

Focussing on the rapid development of implant- and bone-substitute materials as well as their integration in autologous tissue and optimized wound healing processes, it gets more and more important to give well-known therapies new perspectives. For a better adaption of implants in surrounding tissue, material’s biocompatibility and bone regeneration as a part of wound healing becomes subject of scientific research. Many existing therapies on bone regeneration dealing with vitamin D and calcium supplementation are well established (Avenell et al.2014; Javed et al 2016; Vandenbroucke et al 2017). The effect of some other single vitamins or vitamin complexes is already investigated (Masse et al 2010; Elste et al 2017). The effects in direct supplementation with vitamins in bone defects on bone regeneration are not completely understood yet (Owen et al.1990; Herrmann et al 2013)

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