Influence of artificially induced light pollution on the hormone system of two common fish species, perch and roach, in a rural habitat.

Anika Brüning,Werner Kloas,Franz Hölker,Torsten Preuer

doi:10.1093/conphys/coy016

Abstract

Almost all life on earth has adapted to natural cycles of light and dark by evolving circadian and circannual rhythms to synchronize behavioural and physiological processes with the environment. Artificial light at night (ALAN) is suspected to interfere with these rhythms. In this study we examined the influence of ALAN on nocturnal melatonin and sex steroid blood concentrations and mRNA expression of gonadotropins in the pituitary of European perch (Perca fluviatilis) and roach (Rutilus rutilus). In a rural experimental setting, fish were held in net cages in drainage channels experiencing either additional ALAN of ~15 lx at the water surface or natural light conditions at half-moon. No differences in melatonin concentrations between ALAN and natural conditions were detected. However, blood concentration of sex steroids (17β-estradiol; 11-ketotestosterone) as well as mRNA expression of gonadotropins (luteinizing hormone, follicle stimulating hormone) was reduced in both fish species. We conclude that ALAN can disturb biological rhythms in fish in urban waters. However, impacts on melatonin rhythm might have been blurred by individual differences, sampling methods and moonlight. The effect of ALAN on biomarkers of reproduction suggests a photo-labile period around the onset of gonadogenesis, including the experimental period (August). Light pollution therefore has a great potential to influence crucial life history traits with unpredictable outcome for fish population dynamics.

Highlights

Light is fundamental for the existence of flora and fauna on earth
In this study we examined the influence of Artificial light at night (ALAN) on nocturnal melatonin and sex steroid blood concentrations and mRNA expression of gonadotropins in the pituitary of European perch (Perca fluviatilis) and roach (Rutilus rutilus)
E2 and 11-KT levels were measured by enzyme linked immunosorbent assay (ELISA) using commercial kits [RE54021, RE52041, CM582751 (Cayman Chemicals), IBL, Hamburg, Germany]

Summary

Introduction

Light is fundamental for the existence of flora and fauna on earth. It serves as a source of energy and as a source of information to organisms that drives daily and seasonal cycles of behaviour, phenology and physiological change. The natural variation in the length of the day and night sets the internal clock of organisms and ensures that behaviours and physiological processes are synchronized with the time of day and the season (Gaston et al, 2013) This is important in reproduction of many animals, for example, in timing of courtship and mating, which ensures that rearing of offspring can be coordinated with the availability of natural resources, optimizing survival.

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