Influence of artificial CO2 cavity on MMP-2 and VCAM-1, ICAM-1 expression in MDA-MB-231 cell

Abstract

Objective To investigate the influence of in vitro artificial CO2 cavity on matrix metalloproteinase 2(MMP-2), adhesion molecule vascular cell adhesion molecule 1(VCAM-1), and intercellular adhesion molecule 1(ICAM-1)expression in MDA-MB-231 cell. Methods An in vitro artificial CO2 cavity model was established. MDA-MB-231 cells were exposed to CO2 under the pressure of 7 mmHg for 1, 2 and 4 hours, respectively. MMP-2 concentration was measured by enzyme linked immunosorbent assay(ELISA). VCAM-1 and ICAM-1 expression were measured by flow cytometry 0, 24, 48 and 72 hours after CO2-insufflation. Hypoxia group was exposed to 0 mmHg helium for 1 h, and the control group was exposed to 37℃ incubator only. Results Compared with that in the control group, MMP-2 expression in the 1, 2 and 4 hours treatment group was significantly elevated at 0 hour(F=15.045, P<0.05), and the MMP-2 expression in the 2 hours CO2 treatment group was significantly elevated after 24 hours compared with that in the control group and 1, and 4 hours CO2 treatment group(F=5.976, P<0.05). The VCAM-1 expression was significantly elevated at 0 hour and after 24 hours in the 1, 2 and 4 hours CO2 treatment group compared with that in the control group(F1=18.321, F2=20.443, P<0.05), and significantly declined after 72 hours in the 4 hours CO2 treatment group compared with that in 1, and 2 hours CO2 treatment group(F=15.045, P<0.05). ICAM-1 expression was significantly elevated at 0 hour in hypoxia group and 1, 2, 4 hours CO2 treatment group compared with that in the control group, Meanwhile it was higher in 2 hours CO2 treatment group than in 1 hours and 4 hours CO2 treatment group(F=73.765, P<0.05). ICAM-1 expression was significantly elevated after 24 hours in 2 and 4 hours CO2 treatment group compared with that in the control group and 1 hour CO2 treatment group(F=46.322, P<0.05), and it was significantly elevated after 48 hours in 2 hours CO2 treatment group compared with that in the control group and 1, and 4 hours CO2 treatment group(F=22.315, P<0.05). Conclusion The expression of MMP-2, adhesion molecule VCAM-1, and ICAM-1 in MDA-MB-231 cells is elevated after exposure to artificial 7 mmHg CO2 cavity, and CO2 cavity of mastoscopy may modulate the metastasis capacity of breast tumor cells. Key words: Breast neoplasms; Carbon dioxide air pressure; Matrix metalloproteinase 2; Cell adhesion molecules