Abstract
Objective: To evaluate the influence of antisperm antibodies on the acrosome reaction (AR).Design: Clinical study.Setting: University of Marburg, Department of Andrology, Clinical Training Center of the European Academy of Andrology.Patient(s): Spermatozoa from a pool of healthy donors were incubated with 30 seminal plasma samples from infertile men containing antisperm antibodies; they were compared to a control group of 10 samples without antisperm antibodies and five samples with buffer only.Intervention(s): The spontaneous acrosome reaction (SAR) and the induced acrosome reaction (IAR) by calcium ionophore A23187 were observed and determined by means of a flow cytometer. Flow cytometric double-staining estimates of acrosomal integrity were determined by using a monoclonal antibody (TÜS 19), marked with a secondary fluorescein isothiocyanate–labeled antibody. Cell viability was determined by counterstaining with propidium iodide (PI).Main Outcome Measure(s): Number of acrosome reacted spermatozoa.Result(s): The spermatozoa treated with antisperm antibodies showed significantly higher SAR and IAR responses than the control group.Conclusion(s): Some of the antisperm antibodies from individual patients are able to enhance the acrosome reaction in donor sperm, but none of them appeared to inhibit acrosome reaction.
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