Abstract
Tambaqui Colossoma macropomum, Cuvier, 1818 (Osteichthyes: Characidae) were used in this study. The blood of the animals was obtained by punction on the tail vessel with a heparinized syringe (5000 IU) and with one containing EDTA 10%. After collection, the blood was immediately processed in order to measure hematocrit percentage and hemoglobin concentration. It was then left in room temperature where it was kept for a ten-hour period, after which hematocrit and hemoglobin concentrations were measured again. Results indicated that, in teleosts, anticoagulant selection can interfere significantly with the results of the studied hematological parameters. However, blood storage at room temperature did not have a significant effect (α =0.05) on hematocrit percentage, hemoglobin rate or mean corpuscular hemoglobin concentration (MCHC).
Highlights
The present study aims at verifying the effects of EDTA anticoagulants and heparin on hematocrit percentage, hemoglobin rate, and the mean corpuscular hemoglobin concentration (MCHC) in teleosts Colossoma macropomum as well as the effect of blood storage time for this species at room temperature
The results indicated that the hemoglobin rate (0 and 10 hours of storage) in heparinized blood was significantly higher (α=0.05) when compared to samples with EDTA (Figure 1)
The mean percentage of hematocrit obtained with heparin (0 and 10 hours of storage) was considered significantly higher (α=0.05) than that obtained with EDTA (Figure 1)
Summary
To determine the normal hematological parameters in fresh-water teleosts, heparin has been the most frequently anticoagulant used by researchers such as Martins and Pitombeira (1968), Ranzani-Paiva and Godinho (1983), Zuim et al (1984), Ranzani-Paiva and Godinho (1985), Zuim et al (1986), Satake et al (1986, 1991), Ranzani-Paiva (1991), Borghi and Silva-Souza (1992), Wepener et al (1992), Wilhelm-Filho et al (1992), Shiau et al (1993), Martínez et al (1994), Martins et al (1995) and Ranzani-Paiva and Ishikawa (1996) It has been used in hematological variation studies in parasited fish (Ranzani-Paiva et al, 1987). The present study aims at verifying the effects of EDTA anticoagulants and heparin on hematocrit percentage, hemoglobin rate, and the mean corpuscular hemoglobin concentration (MCHC) in teleosts Colossoma macropomum as well as the effect of blood storage time for this species at room temperature
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