Abstract
Abstract Using a kinetic assay, we have examined the role of antibody in the lysis of rabbit erythrocytes (RaRBC) through the alternative complement pathway. Sera from 7 of 11 hypogammaglobulinemic (Hγ) humans and all agammaglobulinemic chickens tested had subnormal activity in the assay. γ-Globulin injections restored normal activity to the two Hγ patients studied. Heated normal human or chicken sera, but not heated Hγ sera, normalized activity in deficient Hγ serum and initiated hemolysis in the presence of rabbit serum as complement source. Absorption of heated normal human serum with RaRBC, but not with sheep erythrocytes or zymosan, removed its ability to reconstitute deficient Hγ serum. Normal hemolytic activity could be restored to Hγ serum with human IgM, IgG, or colostral IgA, with small amounts of goat anti-RaRBC IgG, or with an eluate from serum-sensitized RaRBC, but not with myeloma IgA. Restoration of hemolytic activity to Hγ serum could be achieved in a dose-dependent fashion with the F(ab′)2 fragment of IgG.
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