Abstract
Stages of waste corn cobs processing leading to the production of biohydrogen via dark fermentation are presented and discussed in this paper. Firstly, the effects of pre-treatment conditions i.e. alkaline, alkaline-oxidizing and Fenton oxidizing pre-treatment of lignocellulosic biomass on the removal of lignin were examined. The solid residue obtained in the first stage was subjected to saccharification by means of enzymatic hydrolysis. The composition of enzymatic hydrolysates and fermentation broths were investigated in order to determine the content of sugars as well as phenolic compounds and fermentation broth composition containing organic acids. The dark fermentation process of the obtained hydrolysates was carried using mixed wastewater sludge from the municipal sewage treatment plant. The highest hydrogen production was obtained for alkaline pre-treated hydrolysates. The effects of investigated chemical pre-treatment methods were discussed in terms of the obtained saccharification efficiency and composition of the gas phase formed during the fermentation, and the composition of fermentation broth.
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