Influence of alimentary deprivation on morphofunctional state of the rat's pancreas..

Abstract

Literature data on the effect of alimentary deprivation (AD) on the morphological and functional changes in the pancreas, especially its exocrine part, are rare. The study of morphological changes in the exo- and endocrine pancreas of rats, after exposure to AD, was the aim of our research. Experimental rats received a diet that was reduced in weight by 30%. Access to water was free. The duration of the experiment was 28 days. Rats were decapitated under light ether anesthesia. For histological studies of the pancreas, samples were taken from its central part (body). Morphometric measurements of the gland were carried out using the computer program "Image J". It was revealed that in the exocrine pancreas of adult rats that were on AD, the area of acinus and nucleus of exocrinocytes increased, the number of nucleolus increased, the nuclear-cytoplasmic ratio and the height of the epithelium of the acinus increased. The increase in the activity of the endocrine pancreas, after exposure to AD, indicated by an increase in its following parameters: area (by 108%), number of Langerhans islets (by 34%), their size and number of endocrinocytes (by 55%). Also in the gland of these animals a decreased in the width of the layers of interlobular and interacinus connective tissue by 28%, which improves conditions for the course of metabolic processes. Thus, the effect of AD is accompanied by the appearance of morphological signs of an increased in the activity of both the exocrine and endocrine (to a greater extent) pancreas in adult rats.