INFLUENCE OF AIR ENVIRONMENT CHEMICAL IMPUTITIES ON EXPRESSION OF TOLL-LIKE RECEPTORS AND CYTOKINE LEVELS OF ADOLESCENTS

Abstract

Emissions from oil refining, chemical industry, heat power industry, which are located in the Irkutsk region, cause a high level of air pollution in these areas. Personalized approach to assessing the risk of health disorders allows to set taxis to external influence. The aim of this study was to assess the level of expression of toll-like receptors (TLR) and cytokine content in adolescents with different risk of formation of the immune system disorders caused by the individual inhalant chemical load. Methods. The calculation of the individual chemical load, hazard quotient (HQ) of pollutants and hazard indexes (HI) and dysimmunity for 244 adolescents was carried out based on the data on air pollution and indoor air pollution, the organization of educational process and leisure time, anthropometric and spirometric indices. The content of interleukins-2 and -10, alpha and gamma interferons in the serum of adolescents was studied by the method of enzyme immunoassay. The level of expression of TLR-2 and TLR-4 was assessed by polymeric chain reaction. Results. Persons with an increased risk of developing the immune system pathology were identified as a result of calculating the individual chemical load. It has been stated that the risk of immunity disorders in adolescents living in industrial cities of the Irkutsk region was due to the presence of formaldehyde, nitrogen and sulfur dioxide in the air. While assessing the role of atmospheric and indoor air pollution level in the formation of HQ it was detected that indoor air pollution with formaldehyde was of primary importance. An increase in interleukin-2 and gamma-interferon concentration and decrease in the interleukin-10 concentration was stated with an increase of the air pollution level by chemical impurities that affect the immune system. Conclusion. Thus, the presence of relationships between the indicators of the immune system and increase of their expression with an increase of HI indicates mechanisms contingency of pro-and anti-inflammatory process in the development of adaptive reactions in adolescents under the influence of chemical air pollutants.