Abstract
In this present study, we explored the effects of silver nitrate (AgNO 3) on somatic embryo induction and the development from immature zygotic embryos in Manchurian ash ( Fraxinus mandshurica Rupr.). AgNO 3 played a minor role on in vitro embryo induction frequency and in the number of somatic embryos per explant. However, 1 mg L –1 AgNO 3 enhanced synchronization and significantly inhibited abnormal somatic embryo formation suggesting that AgNO 3 might serve an important function in controlling the development of somatic embryos in Manchurian ash. Our results provided foundation for a future more efficient somatic embryogenesis and regeneration protocol.
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