Abstract
The present study was conducted to investigate the effect of agitation rate on the increase in fresh weight of MD2 pineapple protocorm-like bodies (PLBs) and shoots cultured in liquid medium. PLBs were cultured in 250 ml Erlenmeyer flasks (7 g per flask) containing MS medium and plant growth regulators (1.5 mg/L 6-Benzylaminopurine, BAP and 0.2 mg/L 1-Naphthaleneacetic acid, NAA). The orbital shaker was set at speeds of 50, 80, 100, 120, and 150 rpm. After 40 days, the cultures shaken at 80 rpm showed the highest fresh weight and the highest number of shoots at 76 g and 41 shoots, respectively. A comparative study of agitation found that 80 rpm was the best speed which enhanced both PLB and shoot formation. The findings in the present study would be helpful in setting up large-scale in vitro mass propagation of MD2 pineapple.
Highlights
A lack of plant material is a major problem faced by the Malaysian pineapple industry
The present study was conducted to investigate the effect of agitation rate on the increase in fresh weight of MD2 pineapple protocorm-like bodies (PLBs) and shoots cultured in liquid medium
The aim of the study was to investigate the effect of agitation rate of the orbital shaker on MD2 pineapple propagation and quality, and to compare these results with those obtained with cultures on solid medium
Summary
A lack of plant material is a major problem faced by the Malaysian pineapple industry. This is due to the continued growth of pineapple cultivation areas and the increasing demand for MD2 plant materials. The pineapple is a major tropical fruit in terms of world production [5], with the MD2 variety being among the highly commercialized cultivars especially popular in the European Union market [6]. The National Key Economic Area (NKEA) under Malaysia’s Permanent Food Production Area has listed MD2 as one of the top seven tropical fruits in terms of production output in Malaysia [7]
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