Influence of age on the proliferation of adipose-derived stem cells in vitro

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Objective In order to study the age associated changes,the characteristics of adipose derived stem cells (ADSCs) from 26 to 45 years old healthy volunteers were investigated.Methods Subcutaneous adipose tissue was obtained by tumescent liposuction from healthy women donors.ADSCs were isolated with collagenase digestion.We compared cells from 4 different age groups in 24 female patients ranged from 26 to 45 years old.Characteristics of growth and proliferation capacities of ADSCs were accessed in vitro.ADSCs were characterized by MTT and cell cycle detection methods.Results There was no significant difference of the mononuclear cells count between the four age groups while the first passage time of second and third group were shorter than that of third and fourth group,respectively (P＜0.05).A significant increase in quiescence of S phase was observed in ADSCs from aged donors.ADSCs of third age group showed stronger capability than the fourth one (P＜0.05).The ADSCs MTT activity of fourth group was significantly lower than the previous 3 groups (P＜0.05).Conclusions Proliferation capacities of ADSCs in the group of older than 40 years decrease significantly compared with the younger groups.The results have significantly implicated that proliferation capacities of ADSCs are reduced with aging. Key words: Subcutaneous fat; Adipose-derived stem cells (ADSCs); Age factor; Cell proliferation; Cultured