Abstract
Abstract Exogenous pyruvate was found to have an inhibitory effect on aerobic glycolysis in guinea pig cerebral cortex slices. Under conditions of electrical stimulation, inhibition by pyruvate was not observed. Pyruvate acts as an oxidant by formation of lactate which may explain the observed lower NADH:NAD ratio. This may relieve the NADH inhibition of glyceraldehyde 3-phosphate dehydrogenase, accelerate the removal of fructose 1,6-diphosphate, and cause the defacilitation of phosphofructokinase. This would account for the observation that, in the steady state, pyruvate caused a rise in glucose 6-phosphate and a fall in fructose 1,6-diphosphate concentrations. Omission of phosphate from the tissue medium, although not sufficient to decrease aerobic glycolysis, had a marked effect on the level of glycolytic intermediates and appeared to increase the effects of pyruvate.
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