Abstract
Real–time digestion of type I collagen molecules by bacterial collagenase from Clostridium histolyticum has been monitored using Dynamic Light Scattering method. Time dependencies for translation diffusion coefficient (Dt) and hydrodynamic radius (RH) on were obtained for pure “collagen + collagenase” Tris–HCl buffer solution at different temperatures and for solutions with added CaCl2, ZnCl2 and MgCl2 and EDTA. It was shown that digestion of type I collagen molecules by bacterial collagenase is the first-order reaction. Reaction rate coefficients were calculated.
Highlights
Enzyme-based drugs are commonly used in different fields of modern medicine: in surgery, gynecology, otolaryngology, ophthalmology, as well as in dermatology and cosmetology
We studied the influence of temperature, activators (CaCl2 and ZnCl2) and inhibitors (MgCl2 and EDTA) on collagenase activity
Presented results allow one to draw a conclusion that digestion of type I collagen molecules by bacterial collagenase is the first-order reaction
Summary
Enzyme-based drugs are commonly used in different fields of modern medicine: in surgery, gynecology, otolaryngology, ophthalmology, as well as in dermatology and cosmetology. Clostridium histolyticum collagenases G and H (ColG and ColH) can digest collagens, regardless of their types and sizes [3]. It breaks down multiple bonds in collagen triple helixes as well as peptide bonds [4]. Results obtained with microscopy clearly demonstrate the process of collagen fibers digestion and allow one to evaluate the rate of collagen fibril diameter changing. These results do not agree well with the results obtained in conditions close to physiological [6, 7]. Since collagen by collagenase digestion in solutions takes on average 60 min, DLS enables us to monitor the dynamics of this process in real time [8]
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