Influence of a glycolic acid-based final irrigant for photosensitizer removal of photodynamic therapy on the microhardness and colour change of the dentin structure.

Abstract

This study aimed to evaluate the influence of glycolic acid-based final irrigant for photosensitizer removal of photodynamic therapy on the microhardness and colour change of the dentin structure. Eighty extracted single-rooted human incisors were used. Sample preparation and root split resulted in 160 samples, 80 samples being used for microhardness and 80 samples for colour change evaluation. In the first, PDT protocol was performed and 80 samples were randomly divided into 4 groups (n = 20), according to the final irrigation protocol: distilled water (DW); 17 % ethylenediaminetetraacetic acid (EDTA); QMix; 17 % glycolic acid (GA). Microhardness was evaluated using the Vicker tester, before and after, PDT and final irrigation protocols, calculating the percentage of microhardness reduction. In the second evaluation, PDT and final irrigation protocols were performed in the same way. Colour change was evaluated using digital spectrophotometer before and after these protocols, calculating the ΔE colour change using the CIELAB system (L*a*b* values). Specific statistical analysis was performed for both evaluations (α = 5%). The highest percentage of microhardness reduction was observed in 17 % EDTA, QMix and 17 % GA groups, with no significant difference among them (p > 0.05). Furthermore, none of these protocols was effective in photosensitizer removal, and all final irrigation protocols were statically similar to control group (p > 0.05). GA promotes microhardness reduction and also contributes to the colourization of dentin structure during the photosensitizer removal process, followingPDT .