Influence of 864 MHz electromagnetic field on growth kinetics of established cell line

Abstract

Considering often contradictory data on biological effects of mobile phones frequencies on established cell culture lines, our study aimed at evaluating the influence of 864 MHz electromagnetic field on proliferation, colony forming ability and viability of Chinese hamster lung cells of line V79 cell. Prior to exposure for 1, 2 and 3 hours in transversal electromagnetic mode cell (TEM-cell) cell culture were subcultivated for one day. Cells were exposed to 864 MHz continuous wave (CW) at an average specific absorption rate (SAR) of 0.66 W/kg. Philips PM 5508 connected with signal amplifier was used as signal generator. To determine cell growth, V79 cells were plated in concentration of 1x10/sup 4/cells/ml, and raised in a humified atmosphere at 37/spl deg/C in 5% CO/sub 2/. Cell proliferation was determined by cell counts for each hour of exposure on postexposure days 1, 2, 3, 4 and 5. To identify colony-forming ability, cells were cultivated in concentration of 40 cells/ml, and incubated as above. Colony forming ability for each hour of exposure was defined by colony counts on experimental day 7. Trypan blue exclusion method was used to determine cell viability. In comparison to sham-exposed cells, growth curve of irradiated culture samples showed significant decrease (p<0.05) after 2 and 3 hours of exposure on experimental day 3, respectively. Both, the colony forming ability and viability of irradiated cells did not significantly differ from sham-exposed controls. In conclusion, no vigorous influence of 864 MHz electromagnetic field on growth kinetics of V79 cell culture was detected. Where effect was seen, it was not dose-dependent.