Inflammatory factors and leucocytes are involved in the pathogenesis of malaria associated acute lung injury/acute respiratory distress syndrome in murine model.

Abstract

Event Abstract Back to Event Inflammatory factors and leucocytes are involved in the pathogenesis of malaria associated acute lung injury/acute respiratory distress syndrome in murine model. Michelle K. Sercundes1*, Luana D. Ortolan2, Daniela Debone1, Elizabeth H. Aitken3, José M. Alvarez3, Momtchilo Russo3, Cláudio R. Marinho4 and Sabrina Epiphanio2, 3 1 University of São Paulo, Tropical Medicine, Brazil 2 Federal University of São Paulo, Brazil 3 University of São Paulo, Immunology, Brazil 4 University of São Paulo, Parasitology, Brazil INTRODUCTION: Malaria is a huge burden on global health and caused 2.4 million deaths in 2012. Severe malaria can manifest in the lungs, an illness known as acute lung injury/acute respiratory distress syndrome (ALI/ARDS). Using an experimental model previously established by our group we evaluated the immune cell profile and inflammatory response in malaria associated ALI/ARDS. METHODS AND RESULTS: DBA/2 mice were infected with Plasmodium berghei ANKA. Lungs and bronchoalveolar lavage (BAL) were collected 7 days post infection (7dpi). Using the respiratory patterns (enhanced pause and respiratory frequency) and parasitemia from a survival group, we established ROC curves and were able to classify experimental animals euthanized 7dpi as suffering ALI/ARDS or HP (hyperparasitemia). There was an increase in neutrophils, seen by increased Ncf2 mRNA expression (qRTPCR) and increased numbers of Gr-1high CD11b+ cells (flow cytometry), in the lungs of ALI/ARDS mice compared to HP mice. In the lung we observed increased numbers of alveolar macrophages (F4/80+/CD11C+) and fewer interstitial macrophages (F480+) (flow cytometry) and in the BAL we observed an increase in macrophages and neutrophils (cytospin) in the ALI/ARDS mice. We also found that mRNA expression of iCAM-1, HO-1, IL-10 (anti-inflammatory) and IFNγ (pro-inflammatory) genes was elevated (qRTPCR) in the lungs of the ALI/ARDS mice. CONCLUSION: Our data suggests that neutrophils and alveolar macrophages play a role in ALI/ARDS development. We hypothesize that the neutrophil recruitment is partly dependent on increased ICAM-1 expression and that the high IL-10 mRNA expression is an attempt to re-establish homeostasis. Keywords: Acute Lung Injury, DBA/2 mice, Neutrophils, Inflammatory factors, Acute Respiratory Distress Syndrome Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Sercundes MK, Ortolan LD, Debone D, Aitken EH, Alvarez JM, Russo M, Marinho CR and Epiphanio S (2013). Inflammatory factors and leucocytes are involved in the pathogenesis of malaria associated acute lung injury/acute respiratory distress syndrome in murine model.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00004 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Ms. Michelle K Sercundes, University of São Paulo, Tropical Medicine, São Paulo, Brazil, michellesercundes@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Michelle K Sercundes Luana D Ortolan Daniela Debone Elizabeth H Aitken José M Alvarez Momtchilo Russo Cláudio R Marinho Sabrina Epiphanio Google Michelle K Sercundes Luana D Ortolan Daniela Debone Elizabeth H Aitken José M Alvarez Momtchilo Russo Cláudio R Marinho Sabrina Epiphanio Google Scholar Michelle K Sercundes Luana D Ortolan Daniela Debone Elizabeth H Aitken José M Alvarez Momtchilo Russo Cláudio R Marinho Sabrina Epiphanio PubMed Michelle K Sercundes Luana D Ortolan Daniela Debone Elizabeth H Aitken José M Alvarez Momtchilo Russo Cláudio R Marinho Sabrina Epiphanio Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.